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医学免费论文:诱导的视网膜色素上皮细胞增生作用及其作用机制
【摘要】 目的：观察(R, R)XY10和(S, S)XY10对视网膜色素上皮细胞的增生作用，并且进一步的研究其作用机制。但(R, R)XY10和(S, S)XY10对人脐静脉内皮细胞并无增生的作用。方法：通过人视网膜色素上皮细胞(ARPE19)和人脐静脉内皮细胞(HUVECs)研究(R, R) XY10和(S, S)XY10对视网膜色素上皮细胞的增生作用，并且采用ERK、KT、PI3K、蛋白激酶C(PKC)和一氧化氮合酶(NOS)抑制剂来研究其作用机制。结果：(R, R)XY10和(S, S)XY10促进了ARPE19细胞的增生，并具有剂量依赖性，但是对HUVECs细胞没有影响。如果同时加入增生抑制剂H7 (5μmol/L)、金丝桃素(20μmol/L)、PD98059(2μmol/L)、LY294002(50μmol/L)、SH5(10μmol/L)和LNAME (100μmol/L)，则给予H7、金丝桃素、PD98059和LY294002各组的增生作用受到了抑制，而给予SH5和LNAME两组的增生作用没有影响。结论：(R, R)XY10和(S, S)XY10能够诱导ARPE19细胞增生，其作用可能是通过MAPK和PI3K的途径来发挥该作用。因此，(R, R)XY10和(S, S)XY10能通过修复损伤的RPE细胞来治疗老年性黄斑变性。
【关键词】 年龄相关性黄斑变性;(R,R)XY10; (S,S)XY10; ARPE19 cells;人脐静脉血管内皮细胞;增生
Controlled continuous curvilinear capsulorhexis in short axial length and shallow anterior chamber eyes
GuangYu Yang, YouLi Huang, ZhiFeng Wu 医.学.全.在.线www.med126.com
Department of Ophthalmology, the Second Peoples Hospital,Wuxi 214002,Jiangsu Province,China
Correspondence to:GuangYu Yang.Department of Ophthalmology, the Second Peoples Hospital,Wuxi 214002,Jiangsu Province,China.wuxiygy@163.com
AbstractAIM：To investigate the efficacy of controlled continuous curvilinear capsulorhexis(CCC) technique in short axial length and shallow anterior chamber eyes.METHODS：Sixtyeight patients(68 eyes) with short axial length and shallow anterior chamber were included.The routine CCC technique was used in 32 cases (32 eyes) and controlled CCC technique was used in 36 cases (36 eyes).The success rate and complication were compared between two groups.RESULTS：The success rate of the routine technique group and controlled technique group was 53.13% and 86.11% respectively. Incomplete CCC leading to posterior capsule tears was 9.38% and zero in two groups respectively.CONCLUSION: Controlled CCC technique can increase the success rate and reduce complications in short axial length and shallow anterior chamber eyes.
