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医学论文范文:氧化苦参碱对癫痫大鼠海马组织内ERK信号转导通路及NR2B表达的影响
【摘要】 目的 研究氧化苦参碱(Oxymatrine ,Oxy) 对青霉素致痫大鼠海马内p-ERK 1/2与NR2B表达含量的影响,探讨其抑制癫痫的可能机制。方法 78只SD大鼠随机分为正常组、青霉素(Penicilin,PEN)致痫组、苯巴比妥钠(phenobarbital sodium，PBS)阳性药物对照组和Oxy预处理组,采用免疫组织化学SABC法检测大鼠海马内p-ERK1/2和NR2B阳性神经元在不同时间点的表达。结果 与青霉素模型组比较，Oxy组1.5h大鼠海马内p-ERK 1/2与NR2B 6.0h组阳性细胞数减少( P< 0.01) 。结论 Oxy 拮抗癫痫发作的机制可能与调节癫痫大鼠脑内的p-ERK 1/2水平进而使NR2B的水平降低有关。
【关键词】 氧化苦参碱;癫痫;p-ERK 1/2;NR2B
Effect on ERK signal transduction pathway in hippocampus
of epileptic rats induced by penicillin
ZHAO Yan, ZHANG Lin-na, CHEN Xiao-xia, et al.(Ningxia Medical University, Yinchuan 750004, China)
[Abstract] Objective To investigate the expression of NR2B and extra cellular signal regulated protein kinase (ERK) signal transduction pathway and the significance in the hippocampus of rats after epileptic seizures induced by PEN (penicillin).Methods 78 healthy SD rats were randomly divided into the normal group, a group induced epilepsy by PEN, a group treated by PBS (Phenobarbital sodium) and a group treated by OXY (Oxymatrine). PEN was intraperitoneally injected and established rat's model of epilepsy. Immunohistochemistry method was used to evaluate p-ERK1 / 2 and NR2B protein expression level changes in hippocampus at different time points. Results In the hippocampus of normal group, no immunoreactivity was detected in p-ERK1/2 and a few immunoreactivity of NR2B was observed. Compared with the PEN group, both the p-ERK 1/2 at 1.5h and NR2B at 6.0h express less in hippocampus in OXY group.Conclusion Following PEN -induced seizure, the activations of ERK in rats in the hippocampus changed at different time points. It is possible that OXY inhibit ERK signal pathway thus reduced the expression of NR2B. Therefore OXY changes the process of pathologic and physiologic reactions in PEN-induced epileptic seizure医.学全.在.线网站www.med126.com.
[Key words] Epilepsy; ERK signal transduction pathway; NR2B; Oxymatrine
