参考文献
1.Bigbee JW,et al . Effects of primary antiserum dilution on staining of antlgen rich tissue with the peroxidase antiperoxidase technique. j Histochem Cytochem. 1977；25：433～447
2.Borrsma DM. Preparation of HRP Iabelled antibodies。In Cuello AC(ed) lmmunohistochemistry, John Wiley&,Sons, New York ：Bris－bane Toronto Singapore, 1983：87～100
3.Conchoroff NJ, et al . A monoclonal antibody reactive with 5—Boromo—2 deoxyuridine that does not requireDNA denaturation。Cytometry,1985；6t506～512
4.Costa M, et al. Immunohistochemical localization of polypeptides in peripheral autononic nerves using whole mount preparation, Histo－chemistry, 1980；65：157～165
5.Cumming R. Cyclic nucleotide losses during tissue processing for immunohistochemistry。j HistochemCotychem, 1980j28：54～55
6.Gall JG, Paudue M. Formation and detection of RNA—DNa hybrid molecules in cytological preparations. Proc Natu Acad Sci USA, 1969：63,378～383
7.Grzanna R, Light microscopic immunocytochemistry with fixed unembedded tissue, In Bullock GR&Petrusz P·(eds.)Techniques in immunocytochemistry. London：Academic Press inc, 1982；1：183～204
8.Kohler G, Milstein C. Continuous cultures of fused cells secreting antibody of predefined speciflcity, Nature,1975；256：495～497
9.Imagawa M, et al. Characteristic and evaluation of antibody horseradish peroxidase conjugates prepared by using maleimide compound,glutaraldehyde and periodate. J Biochem, 1982 j 4：41～57
10.Li CY, et al. Use of azide and hydroRen peroxide as an inhibitor for endogenous peroxidase in the immunoperoxidase method , J Histochem Cytochem, 1987；35： 1457～1640
11. Matsuno K, et al. Splenic outer periarterial lymphoid sheath (PALS)：An immunoproliferative microen- vironment constituted by antigen laden marginal metallophils and ED3 positive macrophages in the rat. Cell Tissue Res, 1989；257：459～470
12.Nakane PK, Pierce GB. Enzyme labelled antibodies：Preparation and application for the localization of antigens。j Histochem Cytochem, 1966；14：929～931
13.Norrden SV, Polak JM. Immunocytochemistry today techniques and practice, In Polak JM&Norrden SV(eds.) Immunocytdchemistry. London：Wright PSG Bristol, 1983：11～42
14. Oi VT, Herzenberg LA. Imrnunoglobulin producing hybrid cell lines. In Mishell BB and Shiigi SM, (eds.) Selected methods in cellular immunology, San Francisco：Freeman WH and Company, 1980：351～375
15. Sato Y, et al . The AMeX method. A simplified technique of tissue processing and paraffin embedding with improved preservation of antigens for immunostaining. Am J Pathol, 1986；125：431～435
16.Shi SR, et al . Antigen retrieval in forrnalin fixed,paraffin embedded tissue：An enhancement method for immunohisotchemical staining based on microwave oven heating of tissue sections 。j Histochem Cytochem, 1991；39：741～748
17.Sternberger LA, et al . The unlabelled antibody enzyme method of immunohistochemistry. Preparation and properties of soluble antigen –antigody complex (horseradish peroxidse antihorseradish peroxidase ) and its use in the identification spirochetes, J Hsitochem Cytochem, 1970；18：315～333
18. Sternberger LA, Immunocytochemistry。New York：John Wiley&Sons, 1979
19.Sternberger LA, Conjugated antibody method. In ：Sternberger LA(ed) Immunocytochemistry。3rd edi—tion, New Yor ：John Wiley&Sons, 1986
20. Vacca LL. Double bridge technique of immunocytochemistry.In：Bullock GR&Petrusz P. (eds,) Tech. niques in Immunocytochemistry. London：Academic Press inc, 1982；1：155～182
21. Yasuda K, et al. Application of ultrasound for fixation：Combined use with microwave to enhance the effect of chemical fixation. Acta Histochem Cytochem, 1992；25： 237～244
22. Zhou DS, Komuro T. Interstitial cells associated with the deep muscular plexus of the guinea—pig small intestine, with special reference to the interstitial cells of Cajal. Cell Tissue Res, 1992；268： 205～216
