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医学免费论文:卵巢癌血管生成拟态形态学观察和基质金属蛋白酶表达
【摘要】 目的：在卵巢癌腹腔移植瘤模型中进行卵巢癌血管生成拟态的形态学观察并检测基质金属蛋白酶2(MMP2)的表达情况。方法：卵巢癌细胞SKOV3接种裸鼠腹腔，建立卵巢癌腹腔移植瘤模型，在同一张切片上以抗鼠CD34标记鼠血管内皮细胞，PAS染色行基底膜样结构标记。并应用免疫组化和RT-PCR方法比较MMP2在卵巢癌腹腔移植瘤中央和边缘区域组织的表达差异。结果：卵巢癌腹腔移植瘤中央区域可见卵巢癌细胞围成管状结构，中间可见红细胞，未见CD34阳性细胞出现, PAS阳性物质呈颗粒状弥散分布在卵巢癌细胞浆内，有的贴附在管腔内侧。免疫组化提示MMP2蛋白阳性表达率在肿瘤中央区域组织比肿瘤边缘区域明显增高，RT-PCR分析显示肿瘤组织中央区域MMP2表达与边缘区域相比有显著性差异。结论：卵巢癌细胞分泌PAS阳性物质，可能参与构建肿瘤的血管基质重塑。卵巢癌中央区域可见血管生成拟态，肿瘤中央区域MMP2表达高于肿瘤边缘区域，MMP2表达是否与卵巢癌血管生成拟态形成有关，仍需今后进一步研究。
【关键词】 卵巢癌;血管生成拟态;基质金属蛋白酶2医.学全.在.线网站www.med126.com
Morphologic observation of vasculogenic mimicry and expression of MMP2 in ovarian carcinoma
SU Min1, HUANG Hua2, SUN Feng3, et al (1Department of Gynecology and Obstetrics, 2 Department of Pathology, 3Laboratory of Hematology, Affiliated Hospital of Nantong University, Nantong 226001)
[Abstract] Objective: To determine the formation of vasculogenic mimicry and the expression of matrix metalloproteinase 2(MMP2) in ovarian carcinoma in vivo, after the transplantable tumor model of nude mice was established. Methods: Ovarian carcinoma cells SKOV3 were implanted into peritoneal cavity of nude mice. When the transplantable tumor developed, sections of paraffin-embedded and formaldehyde-fixed tissues were stained with PAS and CD34 to study the morphology and distribution of vasculogenic mimicry. Expression of MMP2 was detected by immunohistochemical staining and RT-PCR in the center and the marginal part of the tumor. Results: Vasculogenic mimicry, when PAS was positive but CD34 was negative, appeared in the center of tumor tissues. Ovarian carcinomatous cells formed patterned network with red blood cells in it. PAS positive substance was distributed over the cytoplasm of ovarian carcinomatous cells and sometimes adhere to the wall of the network. The levels of MMP2 mRNA and protein were higher in the center of the tumor tissue than those in the marginal area. Conclusion: Ovarian carcinomatous cells secrete PAS positive substances, which is related to remodeling vascular matrix in tumor. Vasculogenic mimicry appears in the center of tumor tissues. Expression level of MMP2 in the center of tumor tissues is higher than that in the marginal area. It must be further studied whether MMP2 takes part in formation of vasculogenic mimicry.
[Key words] Vasculogenic mimicry; Ovarian carcinoma;Matrix metalloproteinase 2
在葡萄膜黑色素瘤、乳腺癌、前列腺癌、肝癌、软组织肿瘤以及卵巢癌等高度恶性肿瘤中存在血管生成拟态[1-3]。这些恶性肿瘤细胞在没有血管内皮细胞的参与下直接形成特有的微循环管道，有学者报道通过CD31和PAS双重染色来观察恶性黑色素瘤血管生成拟态的形态学特征[4]。本实验建立裸鼠卵巢癌腹腔移植瘤模型，采用抗鼠CD34标记鼠血管内皮细胞，PAS染色行基底膜样结构标记来进行体内卵巢癌血管生成拟态的形态学观察和定位，并应用免疫组化和RT-PCR法比较基质金属蛋白酶2(matrix metalloproteinase 2，MMP2)在卵巢癌腹腔移植瘤中央和边缘区域组织的表达差异。
