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医学论文范文:CEECL法同时测定复方磷酸可待因中三种成分的方法学研究
【摘要】 目的 建立毛细管电泳电化学发光法分离并检测复方磷酸可待因中三种成分，磷酸可待因、马来酸溴苯那敏和盐酸麻黄碱含量的新方法。方法 基于碱性介质中，复方磷酸可待因溶液中三种主要成分对联吡啶钌在铂电极上的电化学发光信号的增敏作用，且增敏强度和药物浓度成线性关系，与毛细管电泳联用，建立了毛细管电泳电化学发光法分离并检测其含量的新方法。结果 在优化的实验条件下，确定三种药物对照品的线性范围、线性方程和检出限。通过对浓度为1.0×10-5kg/L的磷酸可待因、马来酸溴苯那敏和盐酸麻黄碱对照品进行12次平行测定，其ECL发光强度的RSD值分别为2.89%，3.76%，3.32%。结论 该方法可用于复方磷酸可待因口服溶液中三种主要成分的含量测定，待测样品中的基质不干扰测定，方法的回收率分别为100.4%，101.9%，99.8%(n=5)。
【关键词】 毛细管电泳;电化学发光;磷酸可待因;马来酸溴苯那敏;盐酸麻黄碱
Methodology research on determination of three ingredients in compound codeine phosphate solution by CEECL
LI Haiyan, MUHETAER Tuerhong, TANG Yuhai, ZHANG Guangbin
1. Institute of Analytical Science, College of Sciences of Xian Jiaotong University, Xian 710061; 2. Department of Life Science and Environment Science, Kashi Teachers College, Kashi 844007, China
ABSTRACT: Objective To establish a novel method for simultaneous determination of three ingredients (codeine phosphate, brompheniramine maleate and ephedrine hydrochloride) in compound codeine phosphate solution by using capillary electrophoresis (CE) coupled with electrochemiluminescence (ECL). Methods Based on enhanced ECL intensity of tris (2,2′bypyridine) ruthenium (Ⅱ) with codeine phosphate, brompheniramine maleate and ephedrine hydrochloride, as well as the linear correlation between enhanced sensitivity intensity and drug concentration, platinum disk (300μm in diameter) was used as a working electrode. Parameters affecting separation and detection were optimized. Results Under the optimized conditions, we determined the linear range, regression equation and detection limit for the three ingredients. The relative standard deviation of ECL intensity for twelve consecutive injections of 1.0×10-5kg/L standard sample of three kinds of drug was 2.89% for codeine phosphate, 3.76% for brompheniramine maleate and 3.32% for ephedrine hydrochloride, respectively. Satisfactory results were obtained without interference from sample matrixes. Conclusion The method was successfully applied to the determination of three ingredients in oral solution of compound codeine phosphate, with the recovery rate of 100.4% for codeine phosphate, 101.9% for brompheniramine maleate and 99.8% for ephedrine hydrochloride医.学全.在.线网站www.med126.com.
KEY WORDS: capillary electrophoresis; electrochemiluminescence; codeine phosphate; brompheniramine maleate; ephedrina hydrochloridum
复方磷酸可待因口服溶液具有镇咳祛痰，抗过敏，治疗伤风，流感之功效。其中主要成分为磷酸可待因、盐酸麻黄碱、马来酸溴苯那敏、愈创木酚甘油醚和辅料，成分较为复杂，在一般检测方法中相互干扰和影响。文献中已报道的磷酸可待因的含量测定方法有非水滴定法，酸碱滴定法[1];盐酸麻黄碱的含量测定方法有非水滴定法，分光光度法[1];马来酸溴苯那敏的含量测定方法有非水滴定法[1]，分光光度法[12]，高效液相色谱法[34]，电化学发光法[5]。这些方法都有其不足之处，如混合样品中各成分很难同时检测。本文依据三种待测药物磷酸可待因、盐酸麻黄碱和马来酸溴苯那敏的结构(图1)中的胺基与电化学发光
