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医学论文范文:转录调节因子E26转录因子1蛋白在胎膜早破中的表达和意义
【摘要】 目的 探讨E26转录因子1(E26 transformation specific1, Ets1)蛋白在胎膜早破中的表达和意义。方法 采用免疫组织化学SP法在蛋白水平检测75例胎膜早破者(病例组)及70例无其他产科并发症及内外科合并症的足月择期剖宫产者(对照组)胎膜中Ets1蛋白的表达。结果 ①Ets1蛋白在胎膜滋养层细胞核及胞浆中均有表达，以胞核表达为主;②Ets1蛋白在胎膜早破组滋养层中高表达，与对照组比较，有显著性差异(P<0.05);③Ets1蛋白在未足月胎膜早破组滋养层中高表达，但与足月胎膜早破组比较，无显著性差异(P>0.05)。结论 转录调节因子Ets1在人类胎膜上有表达，且在胎膜早破中高表达，表明Ets1蛋白可能使胎膜细胞外基质重塑从而引发胎膜早破。本研究可作为预测胎膜早破发生的依据。
【关键词】 E26转录因子1;细胞外基质;胎膜早破
Expression and clinical signficance of the transcription factorE26 transformation specific1 in premature
rupture of fetal membranes
GUO Jing, GOU Wenli, SONG Qing
1. Department of Gynecology and Obstetrics, the First Affiliated Hospital, Medical School ofXian Jiaotong University, Xian 710061; 2. Department of Gynecology and Obstetrics,Xian Red Cross Hospital, Xian 710054, China
ABSTRACT: Objective To examine the expression and clinical significance of E26 transformation specific1 in premature rupture of fetal membranes. Methods Fetal membranes from 75 women in the following categories were analyzed for Ets1 expression: preterm and term premature rupture of fetal membranes; 70 women (control group) with term cesarean sections and without complications. Ets1 protein was localized with the use of immunohistochemical SP method. Results Ets1 protein was expressed in both the nucleus and cytoplasm of trophoblast of human fetal membranes, with more obvious expression in the nucleus. Ets1 proteins expression was upregulated in the trophoblast of fetal membranes with premature rupture, which differed significantly from the control group (P<0.05). Ets1 protein's expression was upregulated in the trophoblast of fetal membranes with preterm premature rupture, which did not differ significantly from the control group (P>0.05). Conclusion Ets1 is expressed in human fetal membranes and its expression is upregulated with premature rupture of fetal membranes, suggesting a role for Ets1 in extracellular matrix remodeling of the membranes. This study provides an evidence to predict premature rupture of fetal membrances医.学全.在.线网站www.med126.com.
KEY WORDS: E26 transformation specific1; extra cellular matrix; premature rupture of fetal membrane
胎膜早破(premature rupture of fetal membranes, PROM)是临产前胎膜发生破裂，分为未足月胎膜早破(preterm premature rupture of fetal membranes, PPROM)和足月胎膜早破(term premature rupture of membranes, TPROM)，其发生率国内报道为2.7%～17%，国外报道为5%～15%[1]。且发病率正逐年升高，其中约30%～40%胎膜早破并发早产，为新生儿发病率和病死率上升的一个重要原因。
