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【摘要】 目的 探索一种可以较好地分离口腔双歧杆菌的选择性培养基，以此为基础，检测儿童口腔不同部位双歧杆菌的分布状态，初步探讨口腔双歧杆菌与儿童龋病的发生关系。方法 选择70例3~6岁儿童为研究对象，其中无龋组30例，重症婴幼儿龋(S-ECC)组40例。无龋组收集唾液及唇面、邻面、面沟裂的混合菌斑;S-ECC组收集唾液、光滑面菌斑、牙面白垩斑菌斑和深龋龋坏组织。在经过改良的TPY选择性培养基中进行培养，提取细菌总DNA，用双歧杆菌特异性引物进行聚合酶链反应，对其电泳条带进行记录分析。结果 S-ECC组双歧杆菌的检出率为47.5%，无龋组为0，2组间检出率的差异有统计学意义(P<0.05)。在S-ECC组儿童口腔的不同部位，双歧杆菌检出率分别为唾液27.5%、光滑面菌斑27.5%、白垩斑菌斑20.0%、龋坏组织22.5%，不同检测部位间检出率的差异无统计学意义(P>0.05)。齿双歧杆菌在S-ECC组儿童口腔不同部位的检出率分别为唾液10.0%、光滑面菌斑7.5%、白垩斑菌斑7.5%、龋坏组织10.0%，不同检测部位间检出率的差异也无统计学意义(P>0.05)。结论 改良TPY双歧杆菌选择性培养基是一种适合分离口腔双歧杆菌的选择性培养基;双歧杆菌可能与S-ECC的发生相关，但其检出情况与儿童牙面的部位关系不大。
【关键词】 重症婴幼儿龋; 双歧杆菌; 齿双歧杆菌; 聚合酶链反应
Distribution of Bifidobacterium in oral cavities of children and the relations with caries ZHAI Jing-jing1, ZOU Jing2, LU Li-ying3. (1. State Key Laboratory of Oral Diseases, Sichuan University, Chengdu 610041, China; 2. Dept. of Pediatric Dentistry, West China College of Stomatology, Chengdu 610041, China; 3. Dept. of Pediatric Dentistry, Chinese PLA General Hospital, Beijing 100853, China医.学.全.在.线www.med126.com)
[Abstract] Objective To explore a selected-media of Bifidobacterium from oral cavity, to detect the distribution of Bifidobacterium in different sites of children and primarily investigate the relationship between oral Bifidobacterium and early childhood caries. Methods 70 children aged from 3 to 5-year-old were selected, 30 children were caries-free and 40 were severe early childhood caries(S-ECC). Saliva was collected and plaque samples from the 30 healthy subjects were pooled. For S-ECC group, plaques were collected separately from four different sites as follows: Saliva, surfaces of intact enamel, surfaces of white spot-lesions, and deep dentin-lesions. Samples would be grown in the selected-media, and the whole DNA of bacteria was extracted. Polymerase chain reaction was performed with specific primers and the results were analyzed by the electrophoresis. Results Bifidobacterium were detected 0 in the caries-free children, while 47.5% in the S-ECC group. There was significant difference between two groups(P<0.05) and there was no difference between different sites of teeth in S-ECC group(P>0.05). 27.5% Bifidobacteriumwere detected in saliva, 27.5% on surfaces of intact enamel, 20.0% on surfaces of white spot-lesions and 22.5% in deep dentin-lesions. 10% Bifidobacterium dentium were detected in saliva, 7.5% on surfaces of intact enamel, 7.5%on surfaces of white spot-lesions and 10.0% in deep dentin-lesions. Conclusion One type of modified selected media of Bifidobacterium in oral cavity was explored. Bifidobacterium may be related to the occurrence of the S-ECC andhas nothing to do with different sites of teeth in children.
[Key words] severe early childhood caries; Bifidobacterium; Bifidobacterium dentium; polymerase chain reaction
