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医学免费论文:Galectin3与肺腺癌淋巴及胸膜转移的关系
【摘要】 目的 探讨β半乳糖苷结合蛋白3(Galectin3)表达与肺腺癌淋巴及胸膜转移的关系。方法 采用鼠抗人Galectin3单克隆抗体和免疫组化链霉素抗生物素蛋白-过氧化物酶(SP)法，检测63例肺原发性腺癌和34例胸膜转移性腺癌(均来自肺，影像学显示为非直接侵袭病灶)中Galectin3的表达。结果 发现Galectin3同时存在于胞浆及胞核，未见单独的胞核表达。肺原发性腺癌胞浆、胞核表达阳性率分别为92.1%、31.7%，胞核表达与淋巴结转移(P=0.03)、临床分期有关(P<0.05)。胸膜转移性腺癌胞浆、胞核阳性率分别为97.1%、85.3%，胞核表达明显高于肺原发性腺癌，差异有统计学意义(P<0.05)。结论 细胞核内Galectin3可能增强肺腺癌细胞侵袭转移潜能，可作为判断淋巴结转移和/或胸膜转移的生物学指标。
【关键词】 β半乳糖苷酶类;癌，非小细胞肺;肿瘤转移
pleural metastasis in lung adenocarcinoma医.学全.在.线网站www.med126.com
LONG Chengfeng， LIN Dianjie， CUI Jiyun， WAN Yunyan
(Department of Respiratoty Disease， Provincial Hospital Affiliated to Shandong University， Jinan 250021， China)
To investigate the correlation between galectin3 and lymphatic metastasis and pleural metastasis in adenocarcinoma of the lung. Methods Immunohistochemistry was used to determine expression of galectin3 in 63 primary lung adenocarcinomas specimens and 34 adenocarcinomas metastatic of pleural specimens. Results The patterns of galectin3 expression were cytoplasmic and nuclear. All cases of nuclear galectin3 also showed a cytoplasmic immunoreactivity. In primary tumors the positive galectin3 expression was found in 92.1% of the cytoplasm and 31.7% of the nucleus. Only the nuclear location of galectin3 was associated with lymphatic metastasis (P=0.03) and clinical stages(TNM stages)(P<0.05). In pleural secondary adenocarcinomas the positive expression rate of galectin3 in the cytoplasm and nucleus was 97.1% and 85.3% respectively, and the nuclear staining was significantly higher than the primary tumour(P<0.05). Conclusion Nuclear expression of galectin 3 can enhance the invasive and metastatic potential of lung adenocarcinomas and can be used as a biological marker of lymph node and/or pleural metastasis.
Key words： Betagalactosidase; Carcinoma, nonsmall cell lung; Neoplasm metastasis 在肺癌的研究中发现β半乳糖苷结合蛋白3(Galectin3)多呈高表达，但有关其与肺腺癌淋巴转移及胸膜转移的关系研究报道较少。本研究旨在应用免疫组织化学SP染色方法来检测肺原发性腺癌及胸膜转移性腺癌中Galectin3的表达，初步探讨Galectin3与肺腺癌淋巴结转移及胸膜转移的关系。
1 资料与方法
1.1 资料
1.1.1 临床资料 选取2006年至2007年山东省立医院呼吸内科因胸腔积液原因待查住院患者行内科胸腔镜活检确诊为胸膜转移性腺癌的石蜡标本34例，(来自肺，影像学显示为非直接侵袭病灶)，男15例，女19例，42～64岁，其中影像学或体格检查发现伴淋巴结转移(肿大)21例;另有2007年胸外科手术切除的肺腺癌石蜡标本63例，男29例，女34例，45～74岁，其中伴有淋巴结转移33例。所有病例取标本前未行任何放化疗，均有病例诊断结果。
1.1.2 试剂 实验所用Galectin3鼠抗人单克隆抗体、SP试剂盒(含二抗、三抗工作液)及DAB显示剂均购于福州迈新公司;苏木素购于北京中衫金桥生物公司。
