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医学免费论文:不同时机葛根素治疗对心肌缺血再灌注损伤的保护
【摘要】 目的： 观察缺血前和再灌注前葛根素(PUE)治疗对大鼠心肌缺血-再灌注损伤(I/R)的保护作用。方法： 开胸结扎左冠状动脉前降支(LAD)复制大鼠心肌I/R模型，设立假手术(sham)组、I/R组、PUEA组(缺血前5 min给药)和PUEB组(再灌注前5 min给药)，比色法测定血清肌酸激酶(CK)活力，放射免疫法测定血浆内皮素(ET)浓度，硝酸还原酶法测定血清一氧化氮(NO)浓度，TTC法测定心肌梗死面积。结果： 与I/R组比较，PUEA、B两组血清CK活力明显降低，血浆ET浓度明显降低，血清NO浓度明显增高，心肌梗死面积明显缩小(P<0.05)，且PUEA、B两组间比较差异无显著性(P>0.05)。结论： 缺血前和再灌注前葛根素治疗均能明显减轻大鼠心肌I/R损伤，且两种给药时机的保护效应相近。
【关键词】 葛根素; 心肌再灌注损伤; 肌酸激酶; 一氧化氮; 内皮素
Protective Effect of Puerarin Preconditioning on Myocardial
against Ischemiareperfusion Injury in Rats
LU Deqin, GAO Xiang, LI Baoluo医.学.全.在.线www.med126.com
(Department of pathophysiology, Guiyang Medical College, Guiyang 550004, Guizhou, China)
[Abstract] Objective: To investigate the protective effect of puerarin (PUE) before ischemia or before reperfusion on myocard against ischemiareperfusion injury in rats. Methods: Rats myocardial ischemiareperfusion injury (I/R) were induced by ligating the left anterior descending coronary artery for 45 minutes and reperfusion for 120 minutes. Twentyfour rats were randomly divided into four groups (n=6 each): shamoperation group (group S), ischemiareperfusion group (group I/R), PUE treated groups (group PA received PUE at 100 mg/kg intravenously 5 minutes before ischemia, and group PB received PUE at 100 mg/kg intravenously 5 minutes before reperfusion). Serum creatine kinase (CK) activity was measured with colorimetric method, concentrations of plasma endothelin (ET) detected with radioimmunoassay, and plasma NO content measured with Griess reaction assay. TTC dyeing was used to determine myocardial infarction sizes. Results: CK activity and ET concentrations were significantly lower, and the serum NO contents were significantly higher in groups PA and PB than in group I/R. Myocardial infarct sizes decreased dramatically in groups PA and PB, and there was no significant difference in all of the indexes between the 2 groups. Conclusion: Preconditioning with puerarin before ischemia or before reperfusion can reduce myocardial ischemiareperfusion injury in rats, and the protective effects of puerarin injection at these 2 time points are similar.
[Key words] puerarin; myocardial reperfusion injury; creatine kinase; carbon monoxide; nitric oxide; endothelin
缺血预处理(ischemic preconditioning，IPC)可对缺血-再灌注的脏器产生明显的保护作用，但临床上难以实施，因此药物预处理(pharmacological preconditioning, PPC)正逐渐被人们日益关注。葛根素对心肌缺血-再灌注损伤(ischemiareperfusion injury，I/R)的保护作用已被大量资料证实[1～4]。本课题组也曾报道葛根素预处理对心肌I/R能产生与IPC相似的保护效应[5]，葛根素的保护效应可能与线粒体ATP敏感性钾通道开放有关[6]。2004年3月～2005年1月，采用左冠状动脉前降支(left anterior descending coronary artery，LAD)结扎复制大鼠心肌I/R模型，分别在缺血前和再灌注前给予葛根素治疗，比较不同时机给予葛根素对I/R心肌的保护作用，为临床应用葛根素提供实验依据。
