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医学论文范文:复灌时参附注射液处理可减轻大鼠心肌缺血/再灌注损伤
【摘要】 目的 观察复灌时参附注射液处理对大鼠心肌缺血/再灌注损伤的影响。方法 利用大鼠Langendorff离体心脏灌流模型，制备心肌缺血再灌注损伤模型，在心脏缺血后再灌注同时给予参附注射液，观察大鼠心脏左室舒张末压(LVEDP)、左室发展压(LVDP)、左室内压最大变化速率(±dp/dtmax)和心率(HR),以及心肌组织中的ATP含量、丙二醛(MDA)含量、超氧化物歧化酶(SOD)活性、冠脉流出液中肌酸激酶(CK)含量的变化。实验分为4组：正常对照组、单纯缺血/再灌注组、参附注射液30 ml/L组和60 ml/L组。结果 缺血/再灌注组大鼠心肌ATP含量和SOD活性明显降低，MDA和CK含量明显升高。与缺血/再灌注组比较，参附注射液60 ml/L组心肌MDA值减少，SOD值升高，ATP含量增加，冠脉流出液CK含量减少;血流动力学指标明显改善。结论 复灌时参附注射液处理可提高心肌组织ATP含量和SOD活性，减少MDA生成，减少CK含量，保护缺血/再灌注后的大鼠心脏。
【关键词】 参附注射液;心肌;缺血再灌注
Simultaneous Shenfu injection treatment during reperfusion mitigates ischemia/reperfusion myocardium injury in isolated rat hearts
XU Pengcheng, FENG Haorong, DAI Tijun
1. Jiangsu Provincial Key Laboratory of Anesthesiology, Xuzhou, Jiangsu 221002, China; 2. Department of Anesthesiology, 98th Hospital of PLA, Huzhou, Zhejiang 313000;3. Department of Anesthetic Pharmacology, Xuzhou Medical College, Xuzhou, Jiangsu 221004医学 全在.线提供www.med126.com
Abstract: Objective To investigate the effect of simultaneous treatment with Shenfu injection (SF) during reperfusion on myocardial ischemia/reperfusion injury in rats. Methods The myocardial ischemia/reperfusion model was prepared based on the Langendorff model for the perfusion of isolated rat heart. Following ischemia, reperfusion was allowed with SF injection administered simultaneously. The hemodynamic variables, such as left ventricular end-diastolic pressure (LVEDP), left ventricular developed pressure (LVDP), rate of left ventricular pressure development/relaxation (±dp/dtmax) and heart rate (HR), and the content of adenosine triphosphate (ATP), malendialdehyde (MDA), superoxide dismutase (SOD) in myocardial tissue and the activities of creatine kinase (CK) in coronary effluent fluid were observed. The isolated perfused rat hearts were separated into control, ischemia/reperfusion, SF 30 ml/L and SF 60 ml/L groups. Results In the ischemia/reperfusion group, the content of ATP and activity of SOD significantly decreased; the activities of MDA and CK significantly increased. SF 60 ml/L attenuated the decrease of SOD and the increase of MDA and CK compared with the ischemia/reperfusion group. SF 60 ml/L significantly improved the recovery of the hemodynamic variables and myocardial ATP concentrations following ischemia and reperfusion. Conclusion Simultaneous SF treatment during reperfusion could provide the cardiac protection effect against the ischemia/reperfusion injury by increasing the content of ATP and activity of SOD and decreasing the activity of MDA and CK in myocardial.
Key words: Shenfu injection; myocardium; ischemia/reperfusion
参附注射液是一种中药针剂,由红参和黑附片提取物组成, 主要有效成分为人参皂苷和乌头碱,目前临床已广泛用来治疗心绞痛、心肌梗死、心力衰竭等疾患，也被用于体外循环心脏瓣膜置换术[1-3]。近年来，实验已证实在缺血前应用参附注射液对缺血/ 再灌注损伤心肌有保护作用[4-5]。但复灌时应用参附注射液对心肌缺血/再灌注损伤的作用如何尚不十分清楚，本实验采用Langendorff 大鼠离体心脏灌流模型, 观察复灌后应用参附注射液对离体大鼠心脏缺血/再灌注后血流动力学以及组织中的高能磷酸化合物ATP含量的影响，为临床治疗用药提供实验基础。
