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【摘要】 目的：观察麦冬治疗妊娠糖尿病胰岛素抵抗的作用，研究其作用机制. 方法：选妊娠10 d大鼠，建立妊娠糖尿病胰岛素抵抗动物模型，正常大鼠为对照组，随机将40只妊娠糖尿病胰岛素抵抗大鼠分为4组（n=10)：GDM组、GDM＋麦冬组、GDM＋罗格列酮和GDM＋罗格列酮+麦冬组. 观察用药前后血糖、胰岛素的变化，检测腹腔脂肪组织中TNFα与leptin的mRNA表达变化. 结果：与对照组比较，GDM组大鼠空腹及餐后血糖与胰岛素较正常对照组明显升高（P<0.05)，TNFα与leptin的mRNA表达明显增加（P<0.05)；与GDM组比较，麦冬治疗组、罗格列酮治疗组、罗格列酮+麦冬治疗组大鼠空腹及餐后血糖均下降（P<0.05)，TNFα与leptin的mRNA表达明显降低（P<0.05)；与麦冬治疗组比较，罗格列酮治疗组空腹及餐后血糖无明显变化（P>0.05)，TNFα与leptin的mRNA表达无明显变化（P>0.05)，罗格列酮+麦冬治疗组空腹及餐后血糖明显下降（P<0.01)，TNFα与leptin的mRNA表达明显降低（P<0.05)；与罗格列酮组比较，罗格列酮+麦冬治疗组空腹及餐后血糖明显下降（P<0.01)，TNFα与leptin的mRNA表达明显变化（P<0.05). 结论：麦冬治疗妊娠期糖尿病疗效明显，能变胰岛素抵抗状态，其机制与TNFα与leptin的mRNA表达的变化有关医.学全.在.线网站www.med126.com.
【关键词】 中药;麦冬属；妊娠期糖尿病；胰岛素抵抗；肿瘤坏死因子α；瘦素
基金项目:广东省中医药局资助项目(A2007128)
Treatment effect of Chinese herb Maidong on gestational diabetes mellitus complicated by insulin resistance and its underlying mechanism
LI RuiMan1, QIN JiaJia2, PEI ZhaoHui3
1Department of Obstetrics and Gynaecology, First Affiliated Hospital, 2Department of Obstetrics and Gynaecology of Traditional Chinese Medicine, Medical School, Jinan University, Guangzhou 510630, China, 3Department of Cardiology, First Affiliated Hospital, Sun Yatsen University, Guangzhou 510080, China
【Abstract】 AIM: To investigate the treatment effect of Chinese herb Maidong on gestational diabetes mellitus (GDM) and insulin resistance (IR) and to study its underlying mechanism. METHODS: GDM complicated by IR was induced in rats of 10 gestation days. Normal rats served as a control group. Forty model rats were randomized into GDM group, GDM & Maidong group, GDM & rosiglitazone maleate group, GDM, Maidong & rosiglitazone maleate group (n=10). Serum glucose and serum insulin were determined after and before administration. The expressions of TNFα and leptin were detected in abdominal adipose tissues after and before administration. RESULTS: Compared to controls, serum glucose and serum insulin were evidently increased （P<0.05) and the expressions of TNFα and leptin also significantly rose （P<0.05) in GDM group. Compared to GDM group, serum glucose and serum insulin were significantly decreased （P<0.05) and the expressions of TNFα and leptin declined obviously, too （P<0.05) in GDM & Maidong group, GDM & rosiglitazone maleate group, GDM & Maidong & rosiglitazone maleate group. Compared to GDM & Maidong group, serum glucose and serum insulin remained unchanged （P>0.05)and the expressions of TNFα and leptin were also unchanged （P>0.05) in rosiglitazone maleate group, while serum glucose, and serum insulin （P<0.01) and the expressions of TNFα and leptin were signicantly reduced （P<0.05) in Maidong & rosiglitazone maleate group. Compared to rosiglitazone maleate group, serum glucose and serum insulin were significantly decreased （P<0.05) and the expressions of TNFα and leptin were unchanged （P<0.05) in Maidong & rosiglitazone maleate group. CONCLUSION: Treatment effect of Chinese herb Maidong is good for GDM and can reverse IR. These actions are related to the changes of the contents of TNFα and leptin.
【Keywords】 drugs, Chinese herb; ophiopogon; gestational diabetes mellitus (GDM); insulin resistance (IR); TNFα; leptin
