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医学论文范文:压力超负荷大鼠肾组织脂质过氧化的变化及替米沙坦干预作用
【摘要】目的：探讨腹主动脉缩窄法模拟压力超负荷大鼠模型肾组织脂质过氧化、肾功能改变及替米沙坦干预作用. 方法：将36只Wistar大鼠随机等分成3组: 假手术组(SG)，腹主动脉缩窄组(OG)，替米沙坦+腹主动脉缩窄组(TM＋OG),每组12只. 采用腹主动脉缩窄法制备压力超负荷大鼠模型，分别测定大鼠血清肌酐(Cr)和尿素氮(BUN)含量及大鼠肾组织匀浆丙二醛(MDA)、谷胱甘肽(GSH)含量. 结果：腹主动脉缩窄组(OG)及替米沙坦+腹主动脉缩窄组(TM＋OG)血清Cr, BUN含量及肾组织匀浆MDA水平均较假手术组(SG)增高，但TM＋OG低于OG；肾组织匀浆GSH水平OG及TM＋OG低于SG，但TM＋OG高于OG. 结论：腹主动脉缩窄法制备压力超负荷大鼠肾脏脂质过氧化物改变明显且替米沙坦有保护作用.
【关键词】 肾脏; 压力超负荷; 脂质过氧化; 替米沙坦医.学全.在.线网站www.med126.com
Change of lipid peroxidation of nephric tissue in pressureoverloaded rat model as well as intervention of telmisartan
ZHOU JianGuang1, LIU ZhaoChuan2, MU XianYou2, WANG XuKai3
1Department of Clinical Laboratory, 2Department of Cardiology, Chinese PLA 252 Hospital, Baoding 071000, China,3Department of Cardiology, Daping Hospital, Third Military Medical University, Chongqing 400042, China
【Abstract】 AIM: To illustrate the change in lipid peroxidation of pressureoverloaded rat model produced by abdominal aorta constriction as well as the intervention effects of telmisartan. METHODS: Thirtysix Wistar rats were divided into 3 groups: shamoperation group (SG), abdominal aorta constriction group (operation group, OG), and telmisartan + operation group (TM+OG), with 12 in each group. Pressureoverloaded rat models were established by using abdominal aorta constriction method. The levels of creatinine (Cr) and urea nitrogen (BUN) in the rats serum as well as those of malonaldehyde (MDA) and glutathion (GSH) of the rats nephric tissue were measured respectively. RESULTS: Compared with SG, the levels of Cr and BUN and that of MDA in the nephric tissue were significantly higher than those in OG and TM+OG. The levels of these indices in TM+OG were lower than that in OG. The level of GSH in OG and TM+OG was lower than that in SG, but higher in TM+OG than in OG. CONCLUSION: The change of the lipid peroxidation in the pressureoverloaded rat model is obvious and telmisartan exerts the protective effects against the changes医.学全.在.线网站www.med126.com.
【Keywords】 nephridial tissue; pressure overloads; lipid peroxidation; telmisartan
0引言
心、肾是压力超负荷重要的靶器官，长期压力超负荷可导致心肌细胞肥大，并最终发生慢性心功能不全，心功能不全时肾功能恶化已成为临床工作中的重要问题. 替米沙坦是新一代长效、高效、低毒的新型血管紧张素Ⅱ受体阻滞药. 本文通过腹主动脉缩窄法模拟压力超负荷大鼠模型探讨肾脏脂质过氧化物变化以及血管紧张素Ⅱ受体I型拮抗剂替米沙坦对压力超负荷大鼠肾脏损坏的保护作用.
1材料和方法
