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医学论文范文:加减防己黄芪汤对糖尿病皮肤溃疡大鼠血清NO、ET1水平的影响
【摘要】 [目的]观察加减防己黄芪汤对糖尿病皮肤溃疡大鼠血管内皮功能的影响。[方法]选用SD雄性大鼠90只，随机选取6只为正常对照组，其余大鼠以尾静脉注射四氧嘧啶40mg/kg，选取符合糖尿病诊断标准的大鼠用于建立糖尿病皮肤溃疡模型造模，并随机分成6组，模型对照组、加减防己黄芪汤干预的高、中、低剂量组，中药对照组、西药对照组。实验结束后断头取血检测NO、ET1含量以加减防己黄芪汤对观察对血管内皮功能的影响。[结果]与模型组比较，各治疗组大鼠血清NO含量均有不同程度升高，而血浆ET1含量却有不同程度的降低，其中以加减防己黄芪汤高剂量组变化最明显(P<0.001)，中剂量明显(P<0.01)，低剂量、中药对照组次之(P<0.05)。加减防己黄芪汤各剂量组的调节作用与剂量呈现相关性。[结论]加减防己黄芪汤可通过升高血清NO含量、降低血浆ET1水平来改善血管内皮功能。
【关键词】 糖尿病;皮肤溃疡;一氧化氮;内皮素1;防治;防己黄芪汤
Fund project： Subject supported by Special Foundation of Zhejiang Provincial TCM Modernization (No：Z.C.J.Zi[ 2005 ]241)Effect of Revised Astragalus Tetrandrae Decoction on Rats Serum NO and ET1 with Diabetic Derma Ulcer Huang Zaiwei，Chai Kefu，Song Guangming，et al Zhejiang Chinese Medical University，Hangzhou(310053)
Abstract： [Objective]To explore the cure effect of Astragalus Tetrandrae Decoction on vessel endodermis function of rats with diabetic derma ulcer.[Method]Select 90 SD male rats，take 6 as normal control group，others were injected by tail vein with Alloxan;choose rats in accordance with diabetic diagnosis to set up model of diabetic derma ulcer，and randomly divide them into 6 groups： model control(1)，high，middle and lowdosage groups(2，3，4)of Astragalus Tetrandrae Decoction，TCM control(5)and WM control(6);after test，make decapitated and take blood for NO and ET1 to observe effect of the decoction on vessel endodermis.[Result]Compared to model group，all NO in treated groups was raised in different extent,While plasma ET1 content reduced in different degree;among which，group 2 was the most obvious，then groups 3 and 5;the regulation was related with dosage.[Conclusion]The decoction can improve vessel endodermis function by raising NO and reducing ET1 level医学全.在线www.med126.com.
Key words： diabetes;dermal ulcer;NO;ET1;prevention;Astragalus Tetrandrae Decoction
糖尿病皮肤溃疡发病基础是脾虚失运，浊瘀经络，积久成毒，化腐伤肌。故提出运脾通络法是防治糖尿病足(皮肤溃疡)的有效方法[1]。据此我们以防己黄芪汤为基本方，结合临床化裁出八味中药，制成加减防己黄芪汤。观察该方对糖尿病皮肤溃疡大鼠血清NO、ET1水平的影响，以探讨其作用机制。
1 材料与方法
1.1 实验动物 面军清洁级雄性SD大鼠90只，体重(200±20)g，由浙江省实验动物中心提供，许可证号：SCXK(浙)20030001。
1.2 药物 加减防己黄芪汤(炙黄芪 15g、苍术10g、当归10g、汉防己10g、忍冬藤15g、玄参10g、鬼箭羽10g、威灵仙10g)，由浙江省中药研究所制剂室提供。盐酸二甲双胍片：正安医药(天津)有限公司，批号：060509。糖脉康颗粒：成都中汇制药有限公司，批号：060914。消旋山莨菪碱片：杭州民生药业集团有限公司，批号：107D285。
