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医学论文范文:根除幽门螺杆菌对慢性胃炎和胃溃疡组织中MIF蛋白表达的影响
【摘要】 目的 通过检测幽门螺杆菌(Hp)感染及根除治疗对慢性胃炎和胃溃疡组织中巨噬细胞移动抑制因子(MIF)蛋白表达的影响，探讨MIF和Hp在慢性胃炎和胃溃疡发生发展中的作用。方法 胃镜下随机采集胃黏膜活检组织，经14C呼气试验、Warthinstarry银染色法检测Hp均为阳性，并经病理检验证实为浅表性胃炎、萎缩性胃炎、胃溃疡各40例，Hp阴性的健康检查者25例，采用免疫组织化学SP方法分别检测各组MIF蛋白的表达;进行Hp根治治疗2周，停药4周后复查Hp及MIF，比较根除前后各组MIF水平。结果 MIF蛋白在Hp阴性的正常胃黏膜中阳性表达低(2/25，8%);在Hp感染的慢性浅表性胃炎(12/40，30%)、萎缩性胃炎(26/40，65%)和胃溃疡组织(19/40，47.5%)阳性表达增强，显著高于Hp阴性的正常胃黏膜组(57/120 vs. 2/25;χ2=13.376，P<0.01);在慢性炎症中，随着炎症程度增加，MIF表达增强，差异有显著性(12/40 vs. 26/40;χ2=9.825，P<0.01);Hp根除后，MIF蛋白表达阳性率较治疗前显著降低(57/120 vs. 23/103;χ2=15.264, P<0.01)，而Hp仍为阳性者MIF蛋白阳性率无显著变化。结论 Hp感染导致的胃黏膜慢性炎症及溃疡的形成与胃黏膜MIF的表达相关，根除Hp感染可降低胃黏膜MIF基因表达的异常，可能对预防或减缓胃癌的发生和发展具有重要意义。
【关键词】 巨噬细胞移动抑制因子;幽门螺杆菌;慢性胃炎;胃溃疡
Effect of Helicobacter pylori infection on macrophage migration inhibitory factor protein expression in patients withchronic gastritis and gastric ulcer precancerous lesions
ZHANG Shuangxia, LI Guangdi, YU Xiaohui, ZHANG Fangxin
1. the Second Clinical Medical College of Lanzhou University, Lanzhou 730000;2. Department of Laboratory, the Second Affiliated Hospital of Lanzhou University, Lanzhou 730000; 3. Department of Gastroenterology,Lanzhou General Hospital of Lanzhou Command, Lanzhou 730000, China医.学全.在.线www.med126.com
ABSTRACT: Objective To investigate the effect of Helicobacter pylori (Hp) infection on macrophage migration inhibitory factor (MIF) protein expression and explore the role of Hp and MIF in the development of chronic gastritis and gastric ulcer. Methods The biopsy tissues of gastric mucosa were collected under gastroscope, and Hp was detected by 14C breath test and Warthinstarry method. We recruited 25 healthy people with normal gastric mucosa, 40 patients pathologically confirmed Hppositive with chronic superficial gastritis, 40 with atrophic gastritis and 40 with gastric ulcer. MIF protein expression was examined by immunohistochemical SP staining method, then Hp eradication was performed on Hpinfected chronic superficial gastritis, atrophic gastritis and gastric ulcer for 2 weeks. Hp and MIF were reexamined 4 weeks after drug withdrawal, and difference in MIF expression was compared between Hpinfected patients and Hperadicated patients. Results The expression of MIF was low in normal gastric mucosa without Hp infection (2/25, 8%), but significantly higher in Hpinfected gastric mucosa with chronic superficial gastritis (12/40, 30%), atrophic gastritis (26/40, 65%) and gastric ulcer (19/40, 47.5%); there was a significant difference between normal gastric mucosa without Hp infection and that of Hpinfected patients (57/120 vs. 2/25; χ2=13.376, P<0.01). MIF expression increased with the severity of inflammation in chronic gastritis, and there was a significant difference between superficial gastritis and atrophic gastritis (12/40 vs. 26/40; χ2=9.825, P<0.01). The expression of MIF was noticeably decreased after Hp eradication compared with before(57/120 vs. 23/103; χ2=15.264, P<0.01); however, there was no significant change in those patients whose Hp was still positive. Conclusion The expression of MIF on gastric mucosa is associated with the development of chronic gastritis and gastritis ulcer caused by Hp infection. Eradication of Hp could cut down the abnormally high MIF expression in gastric mucosa and slow down the formation and development of gastric carcinoma.
KEY WORDS: macrophage migration inhibitory factor; Helicobacter pylori; chronic gastritis; gastric ulcer
巨噬细胞移动抑制因子(macrophage migration inhibitory factor, MIF)是重要的炎症因子，能抑制巨噬细胞迁移，促进IV型过敏反应中巨噬细胞的聚积。近年来，许多研究证明MIF参与细胞增殖和分化的调节，在多种炎症性疾病和恶性肿瘤细胞中高表达，在胃癌中的表达显著增高[12]，幽门螺杆菌(helicobacter pylori, Hp)是慢性胃炎、消化性溃疡、胃癌和胃B细胞淋巴瘤重要的危险因子[3]。为进一步探讨Hp和MIF在慢性胃炎胃溃疡向胃癌发生过程中的作用，我们检测了Hp感染的慢性浅表性胃炎、萎缩性胃炎和胃溃疡组织中MIF的表达，并给予Hp根除治疗，分析Hp根除前后MIF表达的变化，探讨MIF和Hp在慢性胃炎和胃溃疡发生发展过程中的可能作用及两者的相关性。
