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医学论文范文:益骨口服液对去势大鼠骨密度与骨生物力学的影响
【摘要】 [目的]探讨益骨口服液对实验去势大鼠骨密度和骨生物力学变化的影响。[方法]将大鼠随机分为A、B、C、D、E五组，A组(10只)为正常对照组(假手术); B组(10只)为模型组(造模后给予蒸馏水亦即空白组);C组(10只)为阳性对照组(切除双侧卵巢并予以倍美力治疗);D组(10只)为高浓度组(造模后给予含生药1.5g/ml益骨口服液治疗);E组(10只)为低浓度组(造模后给予含生药0.5g/ml益骨口服液治疗)。灌胃12周后，活杀各组动物，以双能 X线测定骨密度及三点弯曲试验测生物力学指标。[结果]治疗组骨密度明显高于模型组(P<0.05)，骨生物力学性能较模型组得到明显改善(P<0.05)。结论：益骨汤能促进骨形成，能提高骨强度及生物力学性能。
【关键词】 益骨口服液;骨密度;骨生物力学;去势大鼠
Effect of YiGu Oral Solution on Ovariectomized Rats’ Bone Density and Biomechanics Yao Xinmiao，Xu Luji，Leng Tao et al Third Hospital Affiliated to Zhejiang Chinese Medical University，Hangzhou(310009)
Abstract： [Objective] To observe the effect of YiGu Oral Solution on bone structure and bone biomechanics.[Methods]Rats were randomly divided into A，B，C，D，E five groups，A group(10)for the normal control group(sham operation);B group(10)as a model group(distilled water model that is，after the blank group);C group(10)for the positive control group(resection of bilateral ovarian and western medicine treatment Premarin);D group(10)for the high concentration group(model after treatment with 1.5g/mlYiGu Oral Solution Pharmacognosy );E group(10)for the low concentration group(model after treatment with 0.5g/mlYiGu Oral Solution pharmacognostic).Twelve weeks after oral administration of live animals in each group to kill in order to dualenergy Xray density of bone mineral density determination.[Results]BMD was significantly higher than the model group(P<0.05)，than the mechanical properties of bone biology has marked improvement in the model group(P<0.05).[Conclusion] YiGu Oral Solution can promote bone formation and improve the mechanical properties of bone biology医.学全.在.线www.med126.com.
Key words：YiGu Oral Solution;bone mineral density;bone biomechanics;ovariectomized rats
Fund project： Item supported by Zhejiang Provincial Scitech Bureau(No.2007C33019) 绝经后骨质疏松症是中老年妇女常见的骨代谢性疾病[12]。通过建立去势大鼠骨质疏松症的动物模型，探索益骨口服液防治骨质疏松症的作用机制。补肾健脾是中医治疗此骨质疏松症的方法之一，本实验将以补肾健脾胃为主的中药复方益骨口服液应用于去势大鼠的骨质疏松模型，观察其对大鼠骨密度及骨生物力学的影响。
1 材料与方法
1.1 实验药物 益骨口服液水提液：①按照益骨口服液的提取与成型工艺，由浙江中医药大学实验制剂中心加工。②倍美力水溶液：美国惠氏艾尔斯特制药公司艾尔斯特药厂制造，生产批号0502090，国药准字(2001)J51(2)号。水溶液浓度为9.4ug/ml。③氯氨酮：上海第一生化药业有限公司生产，批号沪卫药准字 (1995)第 001064号。
