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医学论文范文:热休克蛋白701基因多态性与冠心病的关联性研究
【摘要】 目的：探讨热休克蛋白701(HSP701)基因多态性与冠心病的关系。方法：采用病例-对照1:1研究设计。选择湖北省三家综合性医院的217例冠心病患者作为病例组，同时选年龄、性别匹配的217例健康者作为对照组。采用聚合酶链反应-限制性片断长度多态性(PCRRFLP)方法检测HSP701多态性。结果：HSP701基因各基因型及等位基因频率在两组中的分布差异有统计学意义。结论：HSP701基因多态性可能是冠心病的易感基因。
【关键词】 热休克蛋白70 基因多态性 冠心病
Association Study of Heat Shock Protein 701 Gene Polymorphism and Coronary Heart Disease LIU Yong1,2,YANG Handong2^,MIN Xinwen2,LI Dongfeng2 (1Department of Cardiovascular Disease,Renmin Hospital,Wuhan University,Wuhan Hubei 430061;2Department of Cardiovascular Disease,Dongfeng Hospital,Yunyang Medical College, Shiyan, Hubei 442000, China)
Abstract：Objective To explore the association between gene polymorphism of heat shock protein 701(HSP701) and the susceptibility to coronary heart disease. Methods A matched casecontrol study was performed.Two hundreds and seventeen patients with coronary heart disease were selected as case group from three general hospitals in Hubei province,and 217 healthy subjects with age and gender matched were simultaneously selected from the same geographic area as control. HSP701 genotypes were determined with polymerase chain reactionrestriction fragment length polymorphism(PCRRFLP).Results There were significant diferences in the distribution of HSP701 genotypes and allele frequency between case group and control group.Conclusion HSP701 gene polymorphism may be one of genetic susceptibility factors of coronary heart disease.
Key words：Heat shock protein 70;Gene polymorphism;Coronary heart disease
冠心病(coronary heart disease，CHD)是一种常见于40岁以上中老年人的多病因疾病，也是危害职业人群健康最常见的疾病之一。随着冠心病发病率及死亡率的上升，对其病因学方面的研究已成为目前研究的重点和热点，但其发病机制尚不清楚。热休克蛋白(heat shock protein，HSP)又称为应激蛋白，是生物细胞在受热、生物应激、理化因素应激原刺激后，所产生的一类在生物进化中最保守并由热休克基因所编码的伴随细胞蛋白。在许多情况下HSP70在心血管系统优先表达，尤其是在血量丰富的组织，如脑、肝脏、肺脏、心脏等，这说明HSP70与心血管系统有密切的联系。为研究冠心病患者与HSP701基因多态性的相关性本课题进行了初步探索医.学.全.在.线www.med126.com。
1 对象与方法
1.1 研究对象
